Device

Part:BBa_K1886005:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


Constitutive promoter-ho1-pcyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protein ho1 and protein pcyA have to combine with each other to function, so we put them behind the same promoter.



Source

This part is from paper "Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems", and obtained from iGEM team HZAU's lab


References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455. MLA

Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. Tabor, Zachary Booth Simpson, Laura A. Lavery, Matthew Levy, Eric A. Davidson, Alexander Scouras, Andrew D. Ellington, Edward M. Marcotte, Christopher A. Voigt.(2005). Engineering Escherichia coli to see light, Nature,438:441

Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442